Additional file 5 of DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biology

Additional file 5: Table S2. Cost for generating DIMPLE libraries. Listed are the reagents used in generating the Kir2.1 library, the number of these reagents used per sublibrary, their estimated cost based on list prices (not-institutionally agreed discounts) as of July 26 2022, the number of reactions for a 9 sublibrary gene 436 amino acids long such as Kir2.1, and total cost for that reagent across the library. Below is listed the total estimated cost for Kir2.1, $3235.34, and cost per variant, $0.28. Both are likely largely over-estimated for academics due to using list prices. Compared to nicking mutagenesis (0.22$/variant in 2016 excluding QC sequencing) is far more expensive than a comparable gene ($0.15/variant) using DIMPLE.附加文件5：表S2。DIMPLE文库构建成本。本表格收录了构建Kir2.1文库所用的试剂、各亚文库的试剂消耗量、截至2022年7月26日按目录定价（未享受机构协议折扣）计算的试剂预估成本、针对Kir2.1这类长度为436个氨基酸的基因所需的9个亚文库反应次数，以及该试剂在全文库中的总成本。本次统计的Kir2.1文库总预估成本为3235.34美元，单变体成本为0.28美元。由于采用目录定价，针对学术研究者而言，该数值大概率存在显著高估。相较于2016年不含质控（QC）测序的切口诱变技术（单变体成本0.22美元），切口诱变技术的成本远高于使用DIMPLE技术构建同类基因文库的单变体成本（0.15美元）。