遇见数据集

Additional file 15: of Conserved regulation of RNA processing in somatic cell reprogramming

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Figshare2019-02-01 更新2026-04-08 收录
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Table of primers and PCR assays. The XLSX spreadsheet file contains a list of all primers used for the cloning of transgenes into retroviral vectors, qRT-PCR assays for pluripotency, differentiation, epithelial and mesenchymal markers, genomic PCR reactions for transgenes derived from iPSC clones, and Cd44 RT-PCR reactions. (XLSX 7 kb)

引物与聚合酶链式反应(Polymerase Chain Reaction, PCR)实验一览表。本XLSX格式电子表格文件辑录了适配四类实验的全部引物:用于将转基因(transgenes)克隆至逆转录病毒载体(retroviral vectors)的克隆反应、用于检测多能性、细胞分化状态及上皮、间质标志物的定量逆转录PCR(quantitative reverse transcription PCR, qRT-PCR)实验、用于扩增源自诱导多能干细胞(induced pluripotent stem cell, iPSC)克隆的转基因的基因组PCR反应,以及用于Cd44基因检测的逆转录PCR(reverse transcription PCR, RT-PCR)反应。本文件为XLSX格式,大小7千字节。

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2019-02-01
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