Data from: Portable bacteria-capturing chip for direct surface-enhanced Raman scattering identification of urinary tract infection pathogens

Acute urinary tract infections (UTIs) are one of the most common
nosocomial bacterial infections, which affect almost 50% of the population
at least once in their lifetime. UTIs may lead to lethal consequences if
they are left undiagnosed and untreated properly. Early, rapid and
accurate uropathogens detection methods play a pivotal role in clinical
process. In this work, a portable bacteria-grasping surface-enhanced Raman
scattering (SERS) chip for identification of three species of uropathogens
(E. coli CFT 073, P. aeruginosa PAO1, and P. mirabilis PRM1) directly from
culture matrix was reported. The chip was firstly modified with a
positively-charged NH3+ group, which enable itself grasp the
negatively-charged bacterial cells through the electrostatic adsorption
principle. After the bacterial cells were captured by the chip,
concentrated Ag nanoparticles (NPs) were used to obtain their Raman
fingerprint spectra with recognizable characteristic peaks and good
reproducibility. With the help of chemometric method such as discriminant
analysis (DA), the SERS based chip allows a rapid, successful
identification of three species of UTI bacteria with a minimal bacterial
concentration (105 cells/mL) required for clinical diagnostics. In
addition, this chip could spot the bacterial SERS fingerprints information
directly from LB culture medium and artificial urine without sample
pre-treatment. The portable bacteria-grasping SERS based chip provides a
possibility for fast and easy detection of uropathogens, and viability of
future development in healthcare applications.