five

direct RNA seq upon METTL3 KO

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NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/ERP165635
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资源简介:
direct RNA sequencing data sequenced with RNA002 and RNA004 chemistries from control and METTL3 KO mouse embryonic stem cells. Cells culture and treatment (14 days Tamoxifen) and RNA002 library preparation were carried out as in Cruciani et al., 2025 (https://doi.org/10.1186/s13059-025-03498-6). For RNA004, the same RNA was prepared for sequencing according to the the Nanopore direct-rna-sequencing-sqk-rna004-DRS_9195_v4_revB_20Sep2023-promethion protocol. bam files with reads mapped to mm10 are available for: -RNA002 basecalled with m6Abasecaller at https://osf.io/xtnw5/ -RNA004 basecalled with dorado m6A DRACH model at https://osf.io/x4fes/ and https://osf.io/uhr3c/
创建时间:
2026-01-20
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