Flow cytometry data of China

Here, the whole cultivation system was covered with sterile breathable film and acclimated to a 5-day temperature pulse cycle (25 °C for 2 days, 37 °C for 2 days and 25 °C for 1 day) in a controllable temperature incubator under a light intensity of 3000 lux with a 12 h light/12 h dark cycle. Cells reached a plateau after 5 days of growth at 25 °C (Fig. S1). At the end of the first increasing temperature cultivation, a small culture aliquot was inoculated into fresh medium to reinitiate growth for the second increasing temperature treatment. The initial cell density of each increasing temperature treatment was approximately 1 × 104 cells/mL. The control group was exposed to a constant temperature (25 °C for 5 days) for all three stages (Fig. S2). All experiments were performed in three biological replicates. Since the volume of the inoculation solution was much smaller than that of the culture system (&lt;0.01%, v:v), the effect of inoculation on the physical and chemical properties of the culture medium was ignored. At the end of every stage, the number of living and dead cells, mean size (Size/Mean, the average of the forward scattered light intensity) and mean complexity level (Com/Mean, the average of the side scattered light intensity) of each sample were analyzed using a flow cytometer (Accuri C6 CSampler Plus, BD, USA).本实验中，整个培养体系均覆盖无菌透气膜，并于光照强度3000勒克斯、12小时光照/12小时黑暗循环的可控温培养箱中，完成为期5天的温度脉冲循环驯化（25℃维持2天、37℃维持2天、25℃维持1天）。细胞于25℃下培养5天后进入生长平台期（补充图S1）。首轮升温培养结束后，取少量培养等分物接种至新鲜培养基中，以重启生长并开展第二轮升温处理。每一轮升温处理的初始细胞密度约为1×10⁴个细胞/毫升。对照组在全部三个阶段均维持恒温25℃培养（补充图S2）。所有实验均设置3次生物学重复。由于接种液体积远小于培养体系体积（占比<0.01%，体积比），因此忽略接种操作对培养基理化性质的影响。每个阶段结束后，采用流式细胞仪(Flow Cytometer，型号Accuri C6 CSampler Plus，BD公司，美国)对每个样本的活细胞与死细胞数量、平均粒径（Size/Mean，即前向散射光强度的平均值）与平均复杂度（Com/Mean，即侧向散射光强度的平均值）进行分析。