Summary of the IHC, WB, ELISA and bioassay results.

IHC: Score denotes the abundance of PrPd in each visceral tissue; no labelling (−), mild (+), moderate(++) or severe accumulation (+++) of PrPd. For muscle samples a semi-quantitative scoring system was applied to no. of positively labelled spindles; 0 (−), 1 (+), 1–5 (++), >5 (+++)WB: +1/ +2/ +3 denotes pos/neg results from three separate methods where 1 = BioRad; 2 = centrifugal concentration; 3 = NaPTAE = ELISA: +1/ +2 denotes pos/neg results from two separate methods where 1 = BioRad; 2 = IDEXXVB = Vole bioassay results were categorised as follows (+++) = similar to brain dilution-3 or more efficient,(++) = between brain dilutions −3 and −4, (+) = similar to brain dilution −4 or less efficientSummary of the IHC, WB, ELISA and bioassay results.