Summary of the IHC, WB, ELISA and bioassay results.

IHC: Score denotes the abundance of PrPd in each visceral tissue; no labelling (−), mild (+), moderate(++) or severe accumulation (+++) of PrPd. For muscle samples a semi-quantitative scoring system was applied to no. of positively labelled spindles; 0 (−), 1 (+), 1–5 (++), >5 (+++)WB: +1/ +2/ +3 denotes pos/neg results from three separate methods where 1 = BioRad; 2 = centrifugal concentration; 3 = NaPTAE = ELISA: +1/ +2 denotes pos/neg results from two separate methods where 1 = BioRad; 2 = IDEXXVB = Vole bioassay results were categorised as follows (+++) = similar to brain dilution-3 or more efficient,(++) = between brain dilutions −3 and −4, (+) = similar to brain dilution −4 or less efficientSummary of the IHC, WB, ELISA and bioassay results.免疫组化（Immunohistochemistry，IHC）：评分用于表征各内脏组织中致病型朊蛋白（PrPd）的丰度，PrPd沉积情况分为无标记（−）、轻度（+）、中度（++）与重度（+++）四类。针对肌肉样本，采用半定量评分体系对阳性标记肌梭的数量进行评级：0个阳性肌梭记为（−），1个记为（+），1~5个记为（++），多于5个记为（+++）。蛋白质免疫印迹（Western Blot，WB）：+1、+2、+3代表三种独立检测方法的阴、阳性结果，其中1对应伯乐（BioRad）检测法，2对应离心浓缩法，3对应NaPTA法；酶联免疫吸附测定（Enzyme-Linked Immunosorbent Assay，ELISA）：+1、+2代表两种独立检测方法的阴、阳性结果，其中1对应BioRad检测法，2对应IDEXX检测法。田鼠生物测定（Vole bioassay，VB）：结果分类标准如下：(+++) 与脑稀释液-3或更高稀释效率相当，(++) 介于脑稀释液-3与-4之间，(+) 与脑稀释液-4或更低稀释效率相当。本数据集汇总了IHC、WB、ELISA及生物测定的相关结果。