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Micro-flow LC-MS/MS Based Single Shot Proteomics

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NIAID Data Ecosystem2026-03-12 收录
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https://www.omicsdi.org/dataset/pride/PXD023587
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In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run identified about 9,200 proteins from 50 µg of Arabidopsis protein digest. The micro-flow LC-MS/MS system based ssDDA proteomics can be widely used for routine proteomics analysis of large cohort samples.
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2021-07-01
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