Eradiation of leukemia by effective reprogramming-associated cell elimination (ERACE). Mus musculus

We used microarrays to evaluate the global programming of gene expression after Dox inducible Yamanaka factors ectopic expression and identified distinct classes of genes during this biological process in vivo. Overall design: Leukemia cells were sorted from distinct groups at different time points. Microarray was performed at GMINIX in Shanghai. To investigate the molecular mechanism for the elimination of leukemia cells after Dox inducible Yamanaka factors (Oct4,Sox2,Klf4,c-Myc) expression, we sorted GFP+ MLL-AF9 acute myeloid leukemia cells (AML) from leukemic mice and normal Lin-ckit+sca1+ cells (LKS) from 4F transgenic mice. We then performed microarray analysis of these samples before and after Dox-induced OSKM expression or treatment with reprogramming compound cocktail (VPA, CHIR, 616452, TCP) at 24h in vitro.