Westonia WGS vs IWGSC RefSeq v1.0 Genome Assembly

WGS reads of Westonia (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q<30) homozygous; LowQualHet = low quality (Q<30) heterozygous. Files are provided separately for each chromosome part.本数据集包含来自DOI为http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x的研究中Westonia品种的全基因组测序（Whole Genome Sequencing, WGS）读段。使用Minimap2工具将上述读段比对至国际小麦基因组测序联盟（International Wheat Genome Sequencing Consortium, IWGSC）发布的普通小麦（Triticum aestivum）中国春RefSeq v1.0基因组组装版本。 本数据集提供了所有对应的二进制比对映射（Binary Alignment Map, BAM）文件（后缀为.bam）及其BAM索引文件（后缀为.bam.bai），同时附带了测序读段比对覆盖度的bigWig格式汇总文件（后缀为.bam.bw）。 基于上述BAM文件调用单核苷酸多态性（Single Nucleotide Polymorphism, SNP）变异，生成了变异识别格式（Variant Call Format, VCF）文件（后缀为.bam.vcf.gz）及其索引文件（后缀为.bam.vcf.gz.tbi），同时附带了SNP密度（每10千碱基对（kilobase pair, kbp）的SNP数量）的bigWig格式汇总文件（后缀为.bam.vcf.w10000_s10000.bw）。 该VCF文件包含以下过滤标签及其对应含义：PASS代表质量值Q≥30的高质量纯合变异；Het代表质量值Q≥30的高质量杂合变异；LowQualHom代表质量值Q<30的低质量纯合变异；LowQualHet代表质量值Q<30的低质量杂合变异。所有文件均按单个染色体区段分别提供。