Supplementary Material for: Hemolytic Disease of the Fetus and Newborn due to an anti- LU1 (anti-Lua) alloantibody, case report

Many erythrocyte antibodies have been reported to cause hemolytic disease of the fetus and newborn (HDFN), but Lutheran (LU) alloantibodies are normally not involved in HDFN. Here we report the obstetrical history of a 29-year-old women with pregnancy complications due to an anti-LU1 antibody causing fetal anemia and the successful treatment with intrauterine transfusion (IUT). Non-immune hematological causes for fetal anemia and the presence of further alloantibodies, notably antibodies against low frequent antigens, could be ruled out. Swiss national immunohematology reference laboratory in Bern carried out a monocyte monolayer assay (MMA). The monocyte index (MI) was >20%, indicating the presence of a clinical relevant antibody. The diagnosis, acute management, and follow‐up of neonates with fetal anemia still represent an important area of activity for maternity/neonatal services. To our knowledge this is the first reported case of HDFN due to an anti-LU1 antibody requiring IUT.已有诸多研究报道红细胞抗体可引发胎儿和新生儿溶血病（hemolytic disease of the fetus and newborn, HDFN），但路德（LU）血型同种抗体通常并不参与该病的发生。本文报道1例因抗LU1抗体导致胎儿贫血并出现妊娠并发症的29岁女性患者的产科病史，该病例通过宫内输血（intrauterine transfusion, IUT）获得成功治疗。研究人员已排除胎儿贫血的非免疫性血液学病因以及其他同种抗体（尤其是针对低频抗原的抗体）的存在。伯尔尼瑞士国家免疫血液学参考实验室开展了单核细胞单层试验（monocyte monolayer assay, MMA），结果显示单核细胞指数（monocyte index, MI）＞20%，提示存在具有临床意义的抗体。针对胎儿贫血新生儿的诊断、急性期处理及随访仍是产科/新生儿科的重要工作领域。据我们所知，本病例是首例因抗LU1抗体引发胎儿和新生儿溶血病且需行宫内输血的报道病例。