Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

FISH, fluorescence in situ hybridization.a ratio ≥ 2.2.b ratio = 1.8–2.2.c ratio d 3 categories (amplified / equivocal / non-amplified).e 2x) reanalysis Leica FISH confirms amplification Abbott FISH; 1x) new staining Abbott FISH confirms non-amplified score Leica FISH (first Abbott FISH contained 3 biopsies, whereas the biopsy in the middle is missing in the new Abbott FISH and Leica FISH). 2x missing data because no signals were detected with Leica FISH.Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.