Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

FISH, fluorescence in situ hybridization.a ratio ≥ 2.2.b ratio = 1.8–2.2.c ratio d 3 categories (amplified / equivocal / non-amplified).e 2x) reanalysis Leica FISH confirms amplification Abbott FISH; 1x) new staining Abbott FISH confirms non-amplified score Leica FISH (first Abbott FISH contained 3 biopsies, whereas the biopsy in the middle is missing in the new Abbott FISH and Leica FISH). 2x missing data because no signals were detected with Leica FISH.Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.FISH，即荧光原位杂交（fluorescence in situ hybridization）。a 比值≥2.2；b 比值为1.8~2.2；c 比值划分为三类：扩增、可疑、未扩增。2次重新分析：徕卡（Leica）FISH验证了雅培（Abbott）FISH的扩增结果；1次新增染色检测中，雅培FISH判定为未扩增，其对应徕卡FISH评分（首次雅培FISH检测包含3份活检样本，而新增雅培FISH与徕卡FISH检测中均缺失了中间的那份活检样本）。另有2例样本存在缺失数据，因徕卡FISH未检测到杂交信号。本研究针对50例免疫组化（IHC）评分为2+的浸润性乳腺癌组织样本（包括切除标本与活检标本），对比了自动化徕卡HER2 FISH联合数字化分析与手工雅培HER2 FISH联合手工分析的检测结果。