Transgenic Mouse Enhancer Assay - Vista Enhancer ID: mm832

See link to the data preparation protocols for this study in the Additional Information section **Enhancer location:** Mouse genome mm9, chr5: 12505539-12509076 **Name:** mAN261 **Vista Enhancer ID:** mm832 **Nearest craniofacial gene or locus:** Sema3d **Enhancer expression:** positive **Tissues where active:** branchial arch, ear, nose **Comment on OPT files:** This data set includes the following: * 4 movie (.avi) files: A CLIP file that provides an overview of the 3-D model generated by Optical Projection Tomography (OPT)Thresholding-based false-color representation shows unstained regions of the embryo in green, LacZ signal in red. In addition, 3 movies representing a pass-through view of the OPT model in the coronal, sagittal, or transverse plane. * 4 files in .tgz format. Each of these corresponds to one of the .avi files and unzips to a set of .png files, each of which represents the individual frames of the movie (and, in case of the transverse/sagittal/coronal movies, can be used to retrieve individual virtual sections through features of interest in .png image format). [.tgz files can be unzipped with WinZip or Stuffit on a PC or Mac. On linux, treat the file as a gzipped .tar file: gunzip < file.tgz | tar xvf - ]

本研究的数据制备方案详见附加信息章节 **增强子位置：** 小鼠基因组mm9，chr5: 12505539-12509076 **名称：** mAN261 **Vista增强子ID：** mm832 **最近的颅面基因或基因座：** Sema3d **增强子表达：** 阳性 **活性组织：** 鳃弓、耳部、鼻部 **关于OPT文件的说明：** 本数据集包含以下内容：
* 4个AVI格式视频文件：其中1个为CLIP文件，用于展示通过光学投影层析成像（Optical Projection Tomography, OPT）构建的三维模型概览；基于阈值处理的伪彩色标注中，胚胎未染色区域以绿色显示，LacZ信号以红色显示。另外3个视频分别展示OPT模型在冠状面、矢状面或横断面的穿透视图。
* 4个.TGZ格式文件，每个文件对应1个.avi视频文件，解压后将得到一组.png格式文件，分别对应该视频的每一帧；对于冠状面、矢状面及横断面视频，可通过该组文件提取感兴趣区域的虚拟切片，格式为.png图像。[.tgz文件可在PC或Mac设备上通过WinZip或Stuffit工具解压；在Linux系统中，可将其视为gzip压缩的tar文件，使用命令 `gunzip < file.tgz | tar xvf -` 进行解压。]