Dataset related to the article " Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells"

This record contains raw data related to the article "Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells". Adult human cardiac mesenchymal progenitor cells (hCmPC) are multipotent resident populations involved in cardiac homeostasis and heart repair<strong>. </strong>Even if the mechanisms have not yet been fully elucidated, the stem cell differentiation is guided by the mitochondrial metabolism; however, mitochondrial approaches to identify hCmPC with enhanced stemness and/or differentiation capability for cellular therapy are not established. Here we demonstrated that hCmPCs sorted for low and high mitochondrial membrane potential (using a lipophilic cationic dye tetramethylrhodamine methyl ester, TMRM), presented differences in energy metabolism from preferential glycolysis to oxidative rates. TMRM-high cells are highly efficient in terms of oxygen consumption rate, basal and maximal respiration, and spare respiratory capacity compared to TMRM-low cells. TMRM-high cells showed characteristics of precommitted cells and were associated with higher in vitro differentiation capacity throughendothelial, cardiac-like, and, to a lesser extent, adipogenic and chondro/osteogenic cell lineage, when compared with TMRM-low cells. Conversely, TMRM-low showed higher self-renewal potential. To conclude, we identified two hCmPC populations with different metabolic profile, stemness maturity, and differentiation potential. Our findings suggest that metabolic sorting can isolate cells with higher regenerative capacity and/or long-term survival. This metabolism-based strategy to select cells may be broadly applicable to therapies.本数据集收录与《线粒体膜电位差异可区分人心脏间充质祖细胞的不同群体》一文相关的原始数据。成人心脏间充质祖细胞（human cardiac mesenchymal progenitor cells, hCmPC）是参与心脏稳态维持与心脏修复的多能驻留细胞群体。尽管其具体调控机制尚未完全阐明，但干细胞分化进程由线粒体代谢所主导；然而目前尚未建立基于线粒体相关手段，筛选具备更强干细胞干性和/或分化能力、可用于细胞治疗的hCmPC的标准化方法。本研究显示，通过亲脂性阳离子染料四甲基罗丹明甲酯（tetramethylrhodamine methyl ester, TMRM）对线粒体膜电位高低进行分选得到的hCmPC，其能量代谢模式存在显著差异：从偏向糖酵解转变为氧化磷酸化模式。与TMRM低表达细胞相比，TMRM高表达细胞的氧消耗速率、基础呼吸与最大呼吸能力以及储备呼吸容量均显著更高。相较于TMRM低表达细胞，TMRM高表达细胞呈现预定向细胞的特征，且在内皮细胞、类心肌细胞分化方向上表现出更强的体外分化能力，在脂肪细胞、软骨/成骨细胞分化方向上的分化能力相对较弱。反之，TMRM低表达细胞则具备更高的自我更新潜能。综上，本研究鉴定出两种具有不同代谢特征、干细胞成熟度以及分化潜能的hCmPC群体。本研究结果表明，基于代谢特征的细胞分选策略可分离获得具备更强再生能力和/或长期存活能力的细胞。这种基于代谢特征筛选细胞的策略有望广泛应用于各类细胞治疗场景。