The response to a set of potential inducers for the original templates and the 5.3p3c19 clone grown in M9 22 h after induction.

The response is expressed as the % increase in fluorescence when 500 µM of the potential inducer is added compared to the control where only solvent (DMSO) is added. The mean fluorescence was measured for a cell population of 10 000 cells for each sample with the standard error based on at least three independent experiments shown. The basal level of fluorescence (Basal F) is compared to that of WT DntR grown in M9 at the same signal amplification and is measured as % increase in fluorescence for mutant grown with DMSO/fluorescence for wt grown with DMSO. (Wt grown in LB gives a 100%(±30) increase in fluorescence compared to wt grown in M9). SAL: salicylate, DNT: 2,4-dinitrotoluene, 2-NT: 2-nitrotoluene, 2-NB:2-nitrobenzoate, 4-NT: 4-nitrotoluene, 4-NB: 4-nitrobenzoate, BEN: benzoate.本检测结果以荧光增幅百分比形式呈现：当向反应体系中加入500 μM潜在诱导剂时，与仅添加溶剂（二甲基亚砜，DMSO）的对照组相比，计算实验组与对照组的荧光强度相对提升比例。每个样本均对10000个细胞组成的细胞群体测定平均荧光强度，文中展示的标准误基于至少3次独立实验计算得到。本研究中的荧光本底水平（Basal F）以相同信号放大条件下，于M9培养基中培养的野生型（Wild Type, WT）DntR菌株的荧光水平作为参照基准；其荧光增幅百分比通过「（添加二甲基亚砜的突变株荧光强度 - 添加二甲基亚砜的野生株荧光强度）/ 添加二甲基亚砜的野生株荧光强度 × 100%」计算得出。注：于LB培养基中培养的野生株，其荧光强度相较于M9培养基培养的野生株提升100%（±30%）。缩写说明：SAL为水杨酸（salicylate），DNT为2,4-二硝基甲苯（2,4-dinitrotoluene），2-NT为2-硝基甲苯（2-nitrotoluene），2-NB为2-硝基苯甲酸盐（2-nitrobenzoate），4-NT为4-硝基甲苯（4-nitrotoluene），4-NB为4-硝基苯甲酸盐（4-nitrobenzoate），BEN为苯甲酸盐（benzoate）。