Knockdown of RNA deaminase ADAR2 in Th17 cells

ADAR2 is induced upon T cell activation and its immunological function is not known. Here, we performed RNA-seq on Cas9+ control and Th17 cells expressing the PSIN empty vector control or guide RNAs against the ATG start codon or ADAR2 to identify ADAR2 dependent A-to-I edited transcripts. Cultured cells from two independent wildtype mice were used. Results of ADAR2 knockdown (sgAdarb1) Th17 cells were compared to Th17 cells from the same donor transduced with the PSIN empty vector.