Coibamide A Induces mTOR-Independent Autophagy and Cell Death in Human Glioblastoma Cells

Coibamide A is an N-methyl-stabilized depsipeptide that was isolated from a marine cyanobacterium as part of an International Cooperative Biodiversity Groups (ICBG) program based in Panama. Previous testing of coibamide A in the NCI in vitro 60 cancer cell line panel revealed a potent anti-proliferative response and “COMPARE-negative” profile indicative of a unique mechanism of action. We report that coibamide A is a more potent and efficacious cytotoxin than was previously appreciated, inducing concentration- and time-dependent cytotoxicity (EC50科依酰胺A（Coibamide A）是一种经N-甲基稳定的缩酚酸肽（depsipeptide），系从海洋蓝藻中分离得到，其分离工作隶属于总部位于巴拿马的国际合作生物多样性小组（International Cooperative Biodiversity Groups, ICBG）计划。此前，美国国家癌症研究所（National Cancer Institute, NCI）针对科依酰胺A开展的体外60株癌细胞系筛选实验显示，该化合物具有强效抗增殖活性，且呈现"COMPARE阴性"表型，提示其具备独特的作用机制。本研究报道，科依酰胺A的细胞毒素活性较此前认知更为强效且有效，可诱导浓度与时间依赖性的细胞毒性（EC50