Transcriptomic profile of several temperate Cluster A actinobacteriophages during lysogenic growth

The goal of this sequencing project was to measure transcription levels of several Cluster A actinobacteriophages during lysogeny in order to study which genes contribute to lysogeny and superinfection immunity. Total RNA was isolated from cultures grown in log phase, depleted of rRNA and prepared for sequencing using a strand-specific library kit during lysogeny of phages Et2Brutus, Gladiator, and Trixie. A total of three different RNA samples were prepared.