Single cell sequencing data of diabetic and traumatic tibial nerves.

This study enrolled 24 subjects at the Department of Orthopedic Surgery of Shanghai Sixth People’s Hospital, Shanghai, China. Tibial nerves were collected during amputation surgeries in patients suffering from diabetic peripheral neuropathy (DPN) or patients with traumatic limb amputation (TLA). Nerve samples isolated from TLA patients were set as control group. For single-cell RNA sequencing experiments, tibial nerves from donors (4 DPN patients, 3 TLA patients) were rapidly frozen on liquid nitrogen in the operating room and stored until tissue digestion. The sequencing data for 4 DPN samples were named as "DPN_Sample 1-4" and the sequencing data for 3 TLA samples were named as "TLA_Sample 1-3".

本研究于中国上海市第六人民医院骨科纳入24名受试者，在因糖尿病周围神经病变（Diabetic Peripheral Neuropathy, DPN）接受截肢手术，或因创伤性肢体截肢（Traumatic Limb Amputation, TLA）的患者术中采集胫骨神经。其中，从创伤性肢体截肢患者体内分离的神经样本被设为对照组。针对单细胞RNA测序（single-cell RNA sequencing）实验，来自4名DPN患者与3名TLA患者的胫骨神经于手术室中经液氮快速冷冻后保存，直至组织消化步骤。4份DPN样本的测序数据命名为"DPN_Sample 1-4"，3份TLA样本的测序数据命名为"TLA_Sample 1-3"。