Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation

This includes 1- the data set collected at 100mW = 0.04 kW/cm^2 for the Cy3-Cy5 dye pair that is reported in the paper in Figure 2c and SI Figure 14ab 2- Matlab script to do the simplified zeta correction and a Mathematica notebook to show the derivation of simplified zeta correction 3- the raw data corresponding to Figure 2c ----## Citations If you use any of these shared materials, please use the relevant citatations: ``` * Avik K. Pati^{*}, Zeliha Kilic^{*}, Maxwell I. Martin, Daniel S. Terry, Alessandro Borgia, Sukanta Bar, Steffen Jockusch, Roman Kiselev, Roger B. Altman, Scott C. Blanchard, Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation,2024,Nature Methods ``` ----## Relevant Publications In our smFRET data analysis, we used SPARTAN* Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond scale: [paper](https://www.nature.com/articles/nmeth.3769)本数据集包含以下内容： 1. 论文中图2c及补充信息图14ab所报道的、在100mW = 0.04 kW/cm²条件下采集的Cy3-Cy5荧光染料对相关数据集 2. 用于执行简化ζ电势校正的Matlab脚本，以及用于演示简化ζ电势校正推导过程的Mathematica笔记本文件 3. 与图2c对应的原始实验数据 ----## 引用说明 若使用本共享材料，请引用以下相关文献： * Avik K. Pati^{*}, Zeliha Kilic^{*}, Maxwell I. Martin, Daniel S. Terry, Alessandro Borgia, Sukanta Bar, Steffen Jockusch, Roman Kiselev, Roger B. Altman, Scott C. Blanchard, 《从单分子FRET研究中还原真实FRET效率需三重态抑制》, 2024, 《Nature Methods》 ----## 相关研究文献 在我们的单分子福斯特共振能量转移（smFRET, single-molecule Förster resonance energy transfer）数据分析流程中，我们使用了SPARTAN*：《毫秒尺度非平衡分子集合的单分子成像》，[论文链接](https://www.nature.com/articles/nmeth.3769)