Single-cell RNAseq of lamina propria in wild type and LRRK2 G2019S mice after C. rodentium infection

We performed 10X Genomics single-cell RNAsequencing of colonic lamina propria cells from wild type and LRRK2 G2019S mice following 1-week post C. rodentium infection. The cells were pooled from 3 mice per group of both sexes at 8-12 weeks of age. This dataset contains raw FASTQ files from mouse colonic lamina propria, sorted into 4 groups namely wild type and LRRK2 G2019S uninfected and infected mice. Sequencing was performed using NovaSeq 6000 S4 PE 100bp. Reads were processed using the 10X Genomics Cell Ranger Single Cell 2.0.0 pipeline. FASTQs generated from sequencing output were aligned to the mouse GRCm38 reference genome using STAR algorithm 2.7.3a.我们对感染鼠柠檬酸杆菌（C. rodentium）1周后的野生型与LRRK2 G2019S突变型小鼠的结肠固有层细胞，开展了10X基因组学（10X Genomics）单细胞RNA测序。所有样本细胞均取自8-12周龄、雌雄各半的各组小鼠，每组包含3只个体。本数据集包含小鼠结肠固有层细胞的原始FASTQ（FASTQ）文件，按分组分为四类：野生型未感染、野生型感染、LRRK2 G2019S突变型未感染以及LRRK2 G2019S突变型感染小鼠样本。测序采用NovaSeq 6000 S4 PE 100bp平台完成。测序reads的分析处理采用10X基因组学（10X Genomics）Cell Ranger Single Cell 2.0.0流程完成。测序产出的FASTQ文件通过STAR算法（STAR algorithm 2.7.3a）比对至小鼠GRCm38参考基因组。