Diffusion coefficients of free probes, probes hybridized to RNA, and probes hybridized to RNA followed RNaseA/RNaseH digestion.

The fraction hybridized was determined from a two-component fit of the autocorrelation functions. Note that the slight difference from the values in Table 1 is due to the use of a different buffer necessary for RNase treatment and that RNA (Table 1) and probe bound to RNA (Table 2) had virtually identical diffusion coefficients.杂交分数（hybridized fraction）通过自相关函数（autocorrelation functions）的双组分拟合得到。需注意，本结果与表1中数值存在的细微差异，是由于核糖核酸酶（RNase）处理所需的缓冲液不同所致；且表1中的RNA与表2中结合了RNA的探针的扩散系数几乎完全一致。