Direct estimation of clonal mutation rate for Daphnia galeata using three clones (M5, J2 and LC3)

We report here for the first time a directly estimated clonal mutation rate for Daphnia galeata, a widely used model species.We used short term mutation accumulation lines (MAL) for three clones (M5, J2 LC3) and subsequent whole genome sequencing. Data was analyzed for de novo mutations with accuMUlate software. From the total of 18 MAL, we detected 12 single nucleotide mutations in 90 clonal generational passages (0.133 mutations per passage). The haploid SNM rate µ was calculated as 0.745 x 10-9 (95% cf 0.39 x 10-9 – 1.26 x 10-9.