RUNX1 mediating the effects of TGFβ stimulation in cholangiocytes

Cholestatic liver diseases, such as primary sclerosing cholangitis (PSC), are characterized by biliary fibroinflammation. Transforming growth factor-β (TGFβ) activated cholangiocytes release signals that recruit immune cells to drive inflammation and activate myofibroblasts to deposit the extracellular matrix (ECM). TGFβ signaling interacts with RUNX1 transcription factor. However, the role of RUNX1 has not been investigated in biliary fibroinflammation. Here we used mouse large biliary epithelial (MLE) cells that were treated with TGFβ (10ng/mL). ChIP-seq was performed followed by pathway analysis of genes associated with regions of RUNX1 binding. MLE cells were plated to near confluence and treated with TGFβ (10ng/mL) overnight. A RUNX1 polyclonal antibody (ThermoFisher PA5-19638) was used using a ChIP kit (Cell Signaling #900 Simple ChIP Enzymatic Chromatin IP Kit (Magnetic Beads)).