Illumina RNA-Seq, transcript models

Structural annotation/transcript models of RNA (cDNA) sequenced from six different tissues (brain, ileum, lung, ovary, spleen, testis) from the tufted duck on Illumina platform.Raw sequencing data was quality-checked with FastQC, adapters and contamination trimmed with Trimmomatic, quality-checked once again, mapped to the tufted duck reference genome with Hisat2, and transcript models assembled with StringTie2.Trimmomatic options: ${ILLUMINACLIP:TruSeq3-PE_rk.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36}Hisat2 options: ${--dta --rna-strandness RF}Stringtie2 options: ${--fr}