Expression analysis of carlactone producing cyanobacteria

Strigolactones (SLs), plant hormones, are expected to be promising agrochemicals for improving nutrient absorption efficiency by regulating plant-AMF symbiosis. However, the amount of SLs obtained from plants is extremely limited, and there is currently no efficient way to obtain SLs with biological activity. All SLs are converted from a common intermediate, callactone (CL). We have successfully produced CL by using the cyanobacterium Synechococcus elongatus PCC 7942. Syn7942-CL, which expresses the metabolic gene for CL showed slight growth inhibition compared to the vector control strain Syn7942-EV. Thus, RNAseq analysis was carried out to clarify the effect of overexpression of CL synthesis genes.