Identification of target genes of miR-31 in PTC cells by RNA sequencing

The purposes of this study were to compare and analyze the expression differences of papillary thyroid carcinoma (PTC) induced by BrafV600E after knockdown of miR-31 deletion. And in order to gain insights into global gene expression regulated by miR-31 in human PTC cells, RNA-Seq experiments were performed in K1 cells with knockdout of miR-31. mRNA profiles of K1-control and K1-miR-31 KO were generated by deep sequencing, in duplicate, using Illumina novaseq6000 .