Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity

Purpose: mRNAs sequencing of the gene expression differences in LPS-stimulated Extl1-dificient mDC and wild-type mDC. The goals of this study are to investigate the biological effect of glycosyltransferase Extl1 in regulating dendritic cell function. Overall design: Bone marrow cells from Extl1-dificient and wild-type mice were cultured with GM-CSF and IL-4 for 6 days to generate bone-marrow derived DC (BMDC). BMDC were stimulated with or without LPS for 12 h and then subjected to RNA seq.