Phospho-peptides used to generate antibodies against phospho-cGKI species.

Fragments of the N-terminal regions containing in vitro-autophosphorylation sites of cGKIα and cGKIβ were synthesized with phosphorylated residues at the indicated positions (pS, phospho-Ser, pT, phospho-Thr). Three peptide pools (I–III), each containing 2 or 3 phospho-peptides, were used to immunize rabbits (3 rabbits per peptide pool). Peptide sequences were derived from murine cGKIα and cGKIβ, and the amino acids were numbered without counting the N-terminal Met.