QPCR primers for genes of interest (based on Microarray analyses) and reference genes (efficiencies varied between 95.0 and 104.8%).

Genes identified using microarray as being significantly different comparing Soft Tissue Histiocytic Sarcoma (STHS) and Visceral Histiocytic Sarcoma (VHS): C-type lectin domain family 12, member A (CLEC12A); C-C motif chemokine 5 Precursor (Small-inducible cytokine A5) (CCL5_CANFA); Asporin (ASPN); CD9 molecule (CD9);Transketolase-like 1, transcript variant 1 (TKTL1); Complement component 6, transcript variant 1(C6); S100 calcium binding protein A12 (S100A12); Immunoglobulin J polypeptide (IGJ); S100 calcium binding protein A8 (S100A8); Phytanoyl-CoA dioxygenase, peroxisomal like (PHYH)Reference genes primers for q PCR:Hypoxanthine phosphoribosyltransferase (HPRT), Ribosomal protein S19 (RPS19) ribosomalprotein L8 (RPL8), Signal recognition particle receptor (SRPR), Ribosomal protein L13, (RPL13), glucuronidase, beta (GUSB), Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Beta-2-Microglobulin (B2MG), 40S ribosomal protein S5 (RPS5), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-2-microglobulin (B2MG), Ribosomal protein S5 (RPS5).通过基因微阵列（microarray）筛选得到的、在软组织组织细胞肉瘤（STHS）与内脏组织细胞肉瘤（VHS）中表达存在显著差异的基因如下：C型凝集素结构域家族12成员A（CLEC12A）；C-C基序趋化因子5前体（小诱导细胞因子A5）（CCL5_CANFA）；天冬氨酸蛋白聚糖（ASPN）；CD9分子（CD9）；转酮醇酶样1转录变体1（TKTL1）；补体成分6转录变体1（C6）；S100钙结合蛋白A12（S100A12）；免疫球蛋白J多肽（IGJ）；S100钙结合蛋白A8（S100A8）；植烷酰辅酶A双加氧酶（过氧化物酶体样）（PHYH）。实时定量聚合酶链反应（qPCR）用内参基因如下：次黄嘌呤磷酸核糖基转移酶（HPRT）、核糖体蛋白S19（RPS19）、核糖体蛋白L8（RPL8）、信号识别颗粒受体（SRPR）、核糖体蛋白L13（RPL13）、β-葡萄糖醛酸苷酶（GUSB）、甘油醛-3-磷酸脱氢酶（GAPDH）、β2-微球蛋白（B2MG）、40S核糖体蛋白S5（RPS5）、甘油醛-3-磷酸脱氢酶（GAPDH）、β2-微球蛋白（B2MG）、40S核糖体蛋白S5（RPS5）。