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Aspergillus section Nigri: Production of sclerotia on CYA agar or CYA with raisins (most sclerotia were formed around the raisins on the agar surface, and few sclerotia if any on the raisins themselves, see footnotes).

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*-: no sclerotia formed, +: Few scattered sclerotia (10) sclerotia surrounding the raisins, +++: numerous sclerotia formed all over the medium, both with and without raisins.**Homothallic sexual state reported [2], this strain readily produced abundant sclerotia, both with and without raisins.***Paspa is probably an indoloterpene with a paspalinine chromophore.****In this species, sclerotia were only produced on the raisins, not on the medium.*****Abundant sclerotia reported by Musallam [3].Isolates not producing sclerotia in A. brasiliensis, A. niger, and A. welwitschiae and are listed in Table S2 in File S1. The conidia were frozen at −18°C for three weeks before inoculation.

*-:未形成菌核(sclerotia);+:少量分散的菌核(约10个)环绕葡萄干基质;+++:无论是否添加葡萄干基质,培养基全域均形成大量菌核。 **据文献[2]报道,该物种存在同宗配合的有性态,此菌株无论是否添加葡萄干基质,均可大量产生菌核。** ***Paspa大概率为一类带有帕斯卡宁(paspalinine)生色团的吲哚萜类化合物。*** ****在该物种中,菌核仅在葡萄干基质上形成,而非培养基表面。**** *****Musallam[3]曾报道该物种可产生大量菌核。***** 在巴西曲霉(A. brasiliensis)、黑曲霉(A. niger)以及Welwitsch曲霉(A. welwitschiae)中,不产菌核的菌株已在补充文件S1的表S2中列出。供试分生孢子(conidia)于-18℃冷冻三周后再进行接种。

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2015-12-02
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