Histologic features of NASH in serial liver biopsies of both groups of swine.

¶Several percutaneous liver biopsy specimens from baseline were gelatinous and bloody precluding adequate examination*One swine in the control group at week 8 and another swine in the control group at week 24 showed 90% of the hepatocytes). Kupffer cell vacuolization was first observed at week 8, and it became progressively more prominent at weeks 16 and 24. Pericellular fibrosis was first evident at week 16 and it became more prominent at week 24. At week 16, 3 out of 6 swine in the NASH diet group exhibited mild fibrosis and by week 24, 5 out of 6 swine showed moderate fibrosis. Of note, the NASH diet group demonstrated no evidence of significant macrovesicular steatosis or lobular inflammation throughout the dietary intervention. Liver histology of swine in the control group remained unchanged throughout the intervention period.Histologic features of NASH in serial liver biopsies of both groups of swine.基线阶段获取的多份经皮肝穿刺活检标本呈胶状且伴血性，无法进行充分的组织学检查。第8周对照组的1头实验猪与第24周对照组的另1头实验猪，其肝细胞受累比例达90%。库普弗细胞（Kupffer cell）空泡变性于第8周首次被观察到，并在第16、24周逐渐加重。细胞周纤维化于第16周首次显现，且在第24周时病变程度进一步加剧。第16周时，非酒精性脂肪性肝炎（NASH）饮食组6头实验猪中有3头出现轻度纤维化；至第24周，6头猪中有5头表现为中度纤维化。值得注意的是，在整个饮食干预周期内，NASH饮食组未出现显著大泡性脂肪变性或小叶炎症的组织学证据。对照组实验猪的肝脏组织学特征在整个干预期间未发生明显变化。本部分展示两组实验猪系列肝活检标本中的NASH组织学特征。