Mass spectra of plasma apolipoproteins C-I, C-II and C-III and their proteoforms resolved using MSIA loaded with antibodies for apoC-I, C-II and C-III.

Two proteoforms of apoC-I (native and TP truncated), and two proteoforms of apoC-II (native and TQQPQQ truncation) can be detected. 12 different proteoforms of apoC-III can be resolved. This study was focused on four proteoforms of apoC-III: native apoC-III (apoC-III0a), glycosylated apoC-III (apoC-III0b), monosialylated (apoC-III1), and disialylated (apoC-III2).可检测到载脂蛋白C-I（apoC-I）的两种蛋白亚型（天然型与TP截短型），以及载脂蛋白C-II（apoC-II）的两种蛋白亚型（天然型与TQQPQQ截短型）。可分离得到载脂蛋白C-III（apoC-III）的12种不同蛋白亚型。本研究聚焦于载脂蛋白C-III的四种蛋白亚型：天然型载脂蛋白C-III（apoC-III0a）、糖基化载脂蛋白C-III（apoC-III0b）、单唾液酸化载脂蛋白C-III（apoC-III1）以及双唾液酸化载脂蛋白C-III（apoC-III2）。