pH microsensor profiling conducted alongside the destructive sampling of Chesapeake Bay sediments during incubation experiments at Horn Point Lab

These data were collected as part of a sediment incubation experiment, to investigate potential interactions between estuarine cable bacteria and their associated microbial community. Sediments were collected from the main channel of Chesapeake Bay at a mesohaline station that experiences seasonal oxygen depletion. The upper 10 centimeters (cm) of sediment was homogenized under anaerobic conditions, packed into polycarbonate core liners, and incubated in a dark climate-controlled room in aerated aquaria with artificial seawater (S=15.5; T=16 degrees Celsius). In a sub-set of core tubes, a 0.2 micron polycarbonate filter was held embedded at 0.5 cm depth, to block the downward growth of cable bacteria while allowing for porewater diffusion. The sediments (with and without embedded filters) were destructively sampled on 6 dates over 46 days, at which time nucleic acids, samples for microscopy, and samples for porewater geochemistry were collected. Microsensor profiling (oxygen (O2), pH, hydrogen sulfide (H2S)) was conducted alongside the destructive sampling. Associated nucleic acid data are available through NCBI Sequence Read Archive, under Bioproject PRJNA833464. Sediment porewaters were handled anaerobically, and were extracted by centrifugation followed by filtration (0.2 micron in-line filters). Geochemical data include porewater anions (sulfate and chloride) measured by ion chromatography, and ammonium and ferrous iron concentrations measured by standard colorimetric methods. Microscopy data include both counts of cable bacteria measured with FISH oligoprobe DSB706, and total single cell bacteria. The dataset was generated by Pinky Liau under the supervision of Dr. Sairah Malkin, Horn Point Laboratory, U. Maryland Center for Environmental Sciences.

本数据集采集自一项沉积物培养实验，旨在探究河口电缆细菌（cable bacteria）与其相关联的微生物群落间的潜在互作机制。沉积物采自切萨皮克湾（Chesapeake Bay）主河道的一个中盐度站位，该站位存在季节性缺氧现象。将沉积物上层10厘米（cm）在厌氧条件下均质混匀，装填至聚碳酸酯（polycarbonate）芯衬管中，随后置于避光且气候可控的房间内的充气水族箱中培养，培养介质为盐度S=15.5、温度T=16℃的人工海水。在部分芯管中，将0.2微米（μm）的聚碳酸酯滤膜嵌入沉积物0.5 cm深度处，以阻断电缆细菌的向下生长，同时允许孔隙水自由扩散。在46天的实验周期内，共设置6个采样时间点，对含/不含嵌入滤膜的沉积物开展破坏性采样，同步采集核酸样品、显微镜观测样品及孔隙水地球化学样品。破坏性采样同期开展微传感器剖面分析，检测指标包括氧气（O₂）、pH值与硫化氢（H₂S）。相关核酸数据可通过NCBI序列读取档案（Sequence Read Archive）获取，所属生物项目编号为PRJNA833464。沉积物孔隙水在厌氧条件下处理，经离心后采用0.2微米在线滤膜过滤提取。地球化学分析指标包括：孔隙水阴离子（硫酸盐与氯化物）采用离子色谱法测定，铵离子与亚铁离子浓度采用标准比色法测定。显微镜观测数据包含两类：采用荧光原位杂交（FISH）寡核苷酸探针DSB706计数的电缆细菌数量，以及总单细胞细菌总数。本数据集由Pinky Liau在马里兰大学环境科学中心霍恩角实验室Sairah Malkin博士的指导下完成构建。