LAI-1 promotes inactivation of Cdc42 and redistribution of IQGAP1 to the cell cortex.
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(A) A549 cells were treated with LAI-1 (10 μM, 1 h), and the activation state of Cdc42 was analyzed by Western blot using an antibody recognizing Cdc42(GTP/GDP) (left panel). Quantification by densitometry was performed using ImageJ (right panel). A549 cells were treated with LAI-1 (10 μM, 1 h), fixed, stained with antibodies against (B) IQGAP1 or (C) Cdc42 and analyzed by confocal microscopy (left panels; green, FITC; blue, DAPI). The graphs (right panels) are based on the relative fluorescence intensity along cell sections (n = 50, ***p
创建时间：
2016-02-22
