LAI-1 promotes inactivation of Cdc42 and redistribution of IQGAP1 to the cell cortex.

(A) A549 cells were treated with LAI-1 (10 μM, 1 h), and the activation state of Cdc42 was analyzed by Western blot using an antibody recognizing Cdc42(GTP/GDP) (left panel). Quantification by densitometry was performed using ImageJ (right panel). A549 cells were treated with LAI-1 (10 μM, 1 h), fixed, stained with antibodies against (B) IQGAP1 or (C) Cdc42 and analyzed by confocal microscopy (left panels; green, FITC; blue, DAPI). The graphs (right panels) are based on the relative fluorescence intensity along cell sections (n = 50, ***p(A) 将A549细胞以LAI-1（10 μM）处理1小时后，使用识别细胞分裂周期蛋白42（Cdc42）（GTP/GDP结合状态）的抗体，通过免疫印迹法（Western blot）分析Cdc42的激活状态（左图）；采用ImageJ软件通过光密度法完成定量分析（右图）。将A549细胞以LAI-1（10 μM）处理1小时后固定，分别用针对(B) IQ基序GTP酶激活蛋白1（IQGAP1）或(C) 细胞分裂周期蛋白42（Cdc42）的抗体进行免疫荧光染色，通过共聚焦显微镜成像分析（左图：绿色信号对应异硫氰酸荧光素（FITC）标记，蓝色信号对应4',6-二脒基-2-苯基吲哚（DAPI）标记）。右图为沿细胞截面的相对荧光强度统计图（样本量n=50，***p