Summary of data analysis.

*The bottleneck for each biological replicate is calculated by determining the number of insertion sites lost from the input to the output in a set of known neutral genes. The median bottleneck is reported.#The coverage specifies the total number of genes for which fitness values were obtained as a percentage of the total number of non-essential genes in the V. cholerae genome.&A one-sample student's t-test with Bonferroni correction on genes with ≥5 insertion sites was used to determine if the fitness value is statistically different than 1.0.##A student t-test with Bonferroni correction on genes statistically different than 1.0 was used to determine if the fitness value obtained in the test condition is statistically different that the fitness value obtained in the LB grown culture. Additionally, we required at least a 0.15 fitness difference from LB and a fitness value in LB of at least 0.82.*每个生物学重复的瓶颈值，通过统计一组已知中性基因中从输入到输出过程中丢失的插入位点数量计算得到，最终报告中位数瓶颈值。#覆盖度指已获取适应度值的基因总数，以霍乱弧菌（V. cholerae）基因组中非必需基因总数量的百分比进行标注。&针对插入位点数量≥5的基因，采用结合邦费罗尼校正（Bonferroni correction）的单样本学生t检验（one-sample student's t-test），判断其适应度值是否与1.0存在统计学差异。##针对统计学上与1.0存在差异的基因，采用结合邦费罗尼校正的学生t检验（student's t-test），判断测试条件下获取的适应度值与LB培养物中获取的适应度值是否存在统计学差异。此外，本次分析要求两组间适应度差值至少为0.15，且LB组的适应度值不低于0.82。