Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut: supplementary table

Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC–MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma–terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.研究目的：探究选定的鳄鱼肠道细菌是否具有抗菌活性。 材料与方法：实验采用两株分离自湾鳄（Crocodylus porosus）肠道的细菌，分别为铜绿假单胞菌（Pseudomonas aeruginosa）与达卡气单胞菌（Aeromonas dhakensis）。以条件培养基开展针对致病菌的抗菌活性检测，并采用液相色谱-质谱联用（liquid chromatography-mass spectrometry，LC-MS）技术对代谢产物进行分析。 结果与结论：抗菌实验结果显示，条件培养基对革兰氏阳性及革兰氏阴性致病菌均具有强效抑制作用。液相色谱-质谱联用分析共鉴定出210种代谢产物，其中丰度较高的包括：N-乙酰-L-酪氨酸（N-Acetyl-L-tyrosine）、对乙酰氨基酚（Acetaminophen）、反式阿魏酸（Trans-Ferulic acid）、N,N-二甲基甲酰胺（N,N-Dimethylformamide）、邻苯二酚（Pyrocatechol）、环己酮（Cyclohexanone）、苯海拉明（Diphenhydramine）、褪黑素（Melatonin）、γ-松油烯（Gamma–terpinene）、半胱胺（Cysteamine）、3-苯氧基丙酸（3-phenoxypropionic acid）、吲哚-3-甲醇（Indole-3-carbinol）、苯甲醛（Benzaldehyde）、苯佐卡因（Benzocaine）、2-氨基苯甲酸（2-Aminobenzoic acid）、3-甲基吲哚（3-Methylindole）。本研究结果表明，鳄鱼肠道细菌是新型生物活性分子的潜在来源，可作为预防剂、辅助制剂或抗生素，助力人类健康事业发展。