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BackgroundRoux-en-Y gastric bypass（RYGB）surgery delivers an improvement in obesity and obesity-related risks. However, due to the limited operational space in the abdominal cavity of mice, the technical complexity of RYGB surgery and the postoperative complications hinder its mechanism research. The aim was to develop a device that makes it easier to anastomose the esophagus to the jejunum.MethodsWe have invented a simple gastrointestinal anastomosis auxiliary device consisting of a rigid front end and a flexible rear end. Thirty male C57BL6J mice were subjected to RYGB with an auxiliary device. Postoperative recovery and survival status of mice were evaluated using body weight, food intake, body fat, and glucose tolerance.ResultsBased on the RYGB surgical methodology reported in previous literature, the anastomosis device described in this article assists in end-to-end anastomosis of the esophagus and jejunum, which reduces surgical difficulty and time. CT scan results revealed that, following a short - term recovery period after mRYGB surgery, no leakage or stenosis was detected at the anastomotic site in the mice. Moreover, after postoperative recovery, there was no significant difference in food intake, weight and body fat distribution compared with Sham mice, but the glucose tolerance of mRYGB mice was significantly improved.ConclusionsOur modified RYGB surgical method can effectively avoid the problems of anastomotic leakage and stenosis in mice and improve long-term quality of life.背景：鲁氏Y形胃旁路术（Roux-en-Y gastric bypass，RYGB）可改善肥胖及肥胖相关并发症风险。然而，由于小鼠腹腔手术操作空间有限，RYGB手术的技术复杂性及术后并发症阻碍了其相关机制研究的推进。本研究旨在开发一种可简化食管-空肠吻合操作的辅助装置。方法：本研究团队研发了一款结构简易的胃肠道吻合辅助装置，其包含刚性前端与柔性后端。选取30只雄性C57BL6J小鼠，采用该辅助装置开展RYGB手术。通过体重、进食量、体脂及葡萄糖耐量评估小鼠的术后恢复与存活状态。结果：基于既往文献报道的RYGB手术方案，本文所述的吻合装置可辅助完成食管与空肠的端端吻合，降低了手术难度并缩短手术时长。计算机断层扫描（CT）结果显示，小鼠在接受改良式RYGB（mRYGB）术后短期恢复后，吻合部位未出现渗漏或狭窄情况。此外，术后恢复完成后，改良式RYGB小鼠与假手术（Sham）小鼠的进食量、体重及体脂分布无显著差异，但改良式RYGB小鼠的葡萄糖耐量得到显著改善。结论：本研究改良的RYGB手术方法可有效规避小鼠术后吻合口渗漏与狭窄问题，并提升其长期生活质量。