Efficiencies of riPS cell derivation in different culture conditions.
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https://figshare.com/articles/dataset/_Efficiencies_of_riPS_cell_derivation_in_different_culture_conditions_/386550
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To derive rat iPS (riPS) cells 1.5×105 REFs were transduced with the LVTHM-based Oct4, Sox2, Klf4, cMyc, and EGFP lentiviruses. Four days later cells were split onto a 10 cm culture dish and cultured in one of the three reprogramming media. After 10–12 days, primary riPS cell colonies were counted to calculate the reprogramming efficiency. Survival rate was calculated as a relation between the number of picked and the number of surviving clones after 2 passages.
*p<0.05, Student's t-test condition 3 vs condition 2, [DMEM + SR +2i + LIF + A-83-01] N = 3; [N2B27+2i + LIF]: N = 4 independent experiments.
创建时间：
2011-11-04
