ExtFig3A_RACE_INSIG1_SLC7A5_R2_LG292.tiff

<em>Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nucleotides downstream of the predicted cut sites.The PCR products were run on an agarose gel. </em>将Xrn1基因敲除（Xrn1 knock out）的A549细胞分别感染野生型PR8（WT PR8）、PR8-PA(∆X)，或进行模拟感染（mock infected）。随后针对BCAP31、TUBA1B、INSIG1及SLC7A5设计特异性引物，引物结合位点位于各靶基因预测切割位点下游约200~300个核苷酸处，以此开展5'末端快速扩增（5'-Rapid Amplification of cDNA Ends, 5' RACE）实验。将获得的PCR产物进行琼脂糖凝胶电泳检测。