<i>Drosophila miR-263b-5p</i> controls wing developmental growth by targeting <i>Akt</i>

Tissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in <i>Drosophila</i> wing development remains unclear. In this study, we found that the wing size of adult flies was negatively affected by miR-263b expression. The miR-263b-mediated alteration in wing size was linked to a reduction in wing cell number. Additionally, <i>miR-263b</i> overexpression in <i>Drosophila</i> S2 cells decreased cell proliferation and increased cell death. Consequently, we identified <i>Akt</i> as a direct target of miR-263b-5p and found that miR-263b-mediated wing growth regulation was due to changes in <i>Akt</i> expression. Co-expression of <i>Akt</i> in <i>miR-263b</i>-overexpressing wings rescued the <i>miR-263b</i> overexpression-mediated reduction in wing growth. These results enhance our understanding of the crucial role of miRNAs in growth regulation during <i>Drosophila</i> wing development.组织生长受多种信号通路调控，例如胰岛素/胰岛素样生长因子信号（insulin/IGF-signaling, IIS）通路。尽管IIS激活受复杂调控网络的调控，但果蝇（Drosophila）翅发育过程中，基于微小RNA（microRNA，miRNA）的IIS通路调控机制仍未阐明。本研究发现，miR-263b的表达会对成年果蝇的翅尺寸产生负向影响。miR-263b介导的翅尺寸变化，与翅细胞数量减少密切相关。此外，在果蝇S2细胞中过表达miR-263b，会降低细胞增殖速率并促进细胞死亡。本研究证实，Akt是miR-263b-5p的直接靶标，且miR-263b介导的翅生长调控正是通过改变Akt的表达水平实现的。在过表达miR-263b的果蝇翅中共同过表达Akt，可挽救miR-263b过表达所引发的翅生长抑制表型。上述研究结果加深了我们对微小RNA在果蝇翅发育过程中生长调控关键作用的认知。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于果蝇翅膀发育生长调控机制，核心发现是miR-263b-5p通过直接靶向Akt基因来负向调控翅膀大小和细胞数量，揭示了miRNA在胰岛素/IGF信号通路中的关键作用。数据集包含实验数据文件（如图表和表格），支持开放获取（CC BY 4.0许可），适用于发育生物学、分子生物学等领域的研究。