Multiplexed fluorescence imaging based on cycles, raw and processed data.

This dataset was created from a larger acquisition in order to provide an example of reasonnable size, as a companion data set to the F1000Research paper preprint DOIXXX. The original raw data including metadata files are included in <strong>Microscope_Output.zip.</strong> <strong>Experiment.json</strong> and<strong> channelnames.txt </strong>are the ones generated by the acquisition software. They are the only files needed when starting from one of the processed data set below. The deconvolution obtained with the commercial software Microvolution is also provided in <strong>bu_deconvolution.zip.</strong> To start from Step 1(Extended Depth of Field) instead of Step 0 (deconvolution), unzip this file in your output directory and rename the folder bu_deconvolution to out. The extended field of view 2D images created from step 0 to step 2, provided for convenince in <strong>edfonly.zip</strong> The final files generated by trhe Multiplex processor, including the segmentation mask , are provided in<strong> finaloutput.zip</strong>. These files can be used in a specific analysis software.本数据集源自更大规模的采集任务，旨在提供一个规模适中的示例数据集，作为预印本DOI为XXX的F1000Research论文的配套数据集。包含元数据文件的原始数据集已打包至**Microscope_Output.zip**压缩包中。**Experiment.json**与**channelnames.txt**为由采集软件生成的文件，若从下文任一已处理数据集开始处理，仅需这两个文件即可。使用商用软件Microvolution完成的反卷积结果，同样已打包至**bu_deconvolution.zip**压缩包中。若希望从步骤1（扩展景深，Extended Depth of Field）而非步骤0（反卷积）开始处理，请将该压缩包解压至输出目录，并将文件夹bu_deconvolution重命名为out。为便于使用，步骤0至步骤2生成的扩展景深二维图像已打包至**edfonly.zip**压缩包中。由Multiplex处理器生成的最终文件（包含分割掩码，segmentation mask），已打包至**finaloutput.zip**压缩包中。此类文件可在专用分析软件中使用。