Role of TGF-β signaling in hypoxia-induced alternative splicing in breast cancer cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE147516
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Analysis of transcriptome post hypoxia and TGF-β treatment in breast cancer In order to explore the role of TGF-β signaling in mediating the alternative splicing program in breast cancer, we profiled the pre-mRNA splicing and mRNA gene expression upon hypoxia and TGF-β treatment using Human Transcriptome Array 2.0. Breast cancer cell lines MCF7 and HCC1806 were cultured in 1% O2 for giving hypoxia treatment. For TGF-β induction, cells were treated with 10 ng/ml of TGF-β ligand. Post harvest, samples were analysed on Human Transcriptome Array 2.0 platform. Raw files generated were processed using Transcriptome Analysis Console tool.
创建时间:
2020-10-27



