five

piRNA expression in rat ovary. Rattus norvegicus

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA297891
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Purpose: To study the expression profile of piRNAs in rat ovary Methods: Ovary from eight-weeks old, adult wistar rats were harvested after sacrificing the animal. The total RNA is isolated using trizol reagent. Subsequently, small RNA library was contructed using illumina kit as per manufacturer's instructions. Results: The reads were annotated to the rat genome (rn5) and piRNA database and their expression is studied Conclusions: Large number of piRNAs are expressed in rat ovary Overall design: Small RNA profile of rat ovary was generated by deep sequencing using Illumina HiSeq 1000
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2015-10-06
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