Plant physiological and biochemical responses to nickel–cobalt–manganese (NCM) exposure under non-acidified and acidified conditions

This dataset contains raw and processed data obtained from controlled plant bioassays assessing early crop responses to mixed-metal exposure under neutral and acidified conditions. Experiments were conducted using four crop species: maize (Zea mays), wheat (Triticum aestivum), canola (Brassica napus), and Chinese cabbage (Brassica rapa subsp. pekinensis). Two experimental regimes were applied: (i) a non-acidified concentration gradient (0–500 mg·L⁻¹) and (ii) an acidified treatment scenario including an acid control and combined metal–acid treatments (200–1000 mg·L⁻¹). The dataset includes measurements of germination indices, seedling growth traits, tolerance indices, fresh and dry biomass, and photosynthetic pigment contents. Oxidative stress indicators and antioxidant enzyme activities measured under non-acidified conditions are also provided. All measurements were obtained from replicated experiments (n = 3) following standardized physiological and biochemical protocols. The dataset includes raw replicate values and processed summary statistics (mean ± standard error) used for statistical analyses and figure preparation in the associated manuscript.本数据集包含来自受控植物生物测定的原始与处理后数据，该测定用于评估作物在中性及酸化条件下对混合金属暴露的早期响应。实验共选用4种作物：玉米（Zea mays）、小麦（Triticum aestivum）、油菜（Brassica napus）以及大白菜（Brassica rapa subsp. pekinensis）。 本研究设置两类实验处理体系：(i) 非酸化浓度梯度组（0–500 mg·L⁻¹）；(ii) 酸化处理组，包含酸化对照组与金属-酸复合处理组（200–1000 mg·L⁻¹）。本数据集涵盖萌发指数、幼苗生长性状、耐性指数、鲜干生物量及光合色素含量的测定数据，同时提供非酸化条件下测定的氧化应激指标与抗氧化酶活性数据。 所有测定数据均来自遵循标准化生理生化实验方案的重复实验（n=3）。本数据集包含原始重复测定值与经处理的汇总统计量（平均值±标准误），可用于相关论文中的统计分析与图表制作。