miRNA microarray of rTDSCs under mechanical treatment or not. miRNA microarray of rTDSCs under mechanical treatment or not

Tendinopathy is a common disease in individuals whose tendons suffer from repetitive strains, such as athletes. The lack of knowledge regarding the pathological mechanisms causes inadequate prevention methods and low cure rate of this disease. Repetitive strain injury is thought to be the source of tendinopathy; therefore, we investigate the effect of mechanical loading on tendon and seek the ways to prevent and treat tendinopathy better. In our study, we found that the expression of a mechanosensitive miRNA, miR-337-3p, decreased in tendon-derived stem cells (TDSCs) under uniaxial cyclical mechanical loading with 10% elongation. In addition, miR-337-3p was negatively related to chondro-osteogenic differentiation of TDSCs, in which lower expression was found in human calcified tendons and rat tendinopathy samples. Overall design: 3 samples of rTDSCs untreated as control, and 3 samples of rTDSCs under mechanical loading for 7days of 10% elongation,0.5Hz, 8h per day as treatment group