RNA-seq profiling of LPS-treated vs un-treated primary monocytes derived from rhesus macaques and sooty mangabeys

To characterize more broadly the effect of attenuated TLR4 signaling responses in sooty mangabeys, we performed a comparative RNA-seq profiling of LPS-treated primary monocytes from rhesus macaques and sooty mangabeys. Production of TNF- and IL6 mRNA was significantly lower in sooty mangabeys. Moreover, we observed that induction of NF-κB -regulated inflammatory genes was broadly and significantly reduced in sooty mangabeys, for TNF-a and IL-6 signaling pathways, compared to rhesus macaques. Overall, these data indicate that LPS stimulation of SM blood cells results in a significantly blunted production of pro-inflammatory cytokines as compared to rhesus macaque macrophages. Examination of LPS-treated and un-treated primary monocytes derived from 6 rhesus macaques and 6 sooty mangabeys for a total of 24 samples.