TXNIP loss increases Myc genomic occupancy [ChIP-seq]

Our RNA sequencing result demonstrated that TXNIP loss increased the levels of Myc-dependent transcription. To determine whether TXNIP regulates global Myc genomic occupancy, we performed Myc ChIP-seq on parental 231 and 231:TKO cells. Overall design: TXNIP was knocked out in MDA-MB-231 cells using CRISPR-Cas 9 method. Parental MDA-MB-231 (parental 231) cells and TXNIP knockout MDA-MB-231 (TKO) cells were used to perform Myc ChIP sequencing.